Sequence Data module

Using the Sequence Data module, one can assemble sequence reads ranging from single genes obtained via classical Sanger sequencing to full genomes via Next Generation Sequencing (NGS). In addition, the BIONUMERICS software recognizes widely used sequence file formats such as EMBL, GenBank, and FASTA, and stores the full header and feature information of annotated sequences.

Sequence editor in the BIONUMERICS software

With the addition of the Sequence Data module, BIONUMERICS becomes a rich and uniquely integrated sequence analysis software, offering a variety of analysis tools such as multiple sequence alignment, phylogenetic clustering, chromosome comparison and alignment, automated annotation, chromosome-wide SNP analysis, PCR primer design, restriction enzyme analysis, open reading frame analysis, etc.

BIONUMERICS' powerful database back end supports sequence analysis in high throughput projects. Furthermore, available plugins, script tools and pipeline engines allow for reliable automated batch processing of massive amounts of sequence data.


Multiple sequence alignment

BIONUMERICS offers probably the finest and most comprehensive multiple sequence alignment tool that currently exist for PCs. It combines clustering of thousands of nucleotide or protein sequences of almost unlimited length with multiple alignment and display of homology matrices.

Open reading frame analysis

Frame analysis finds all open reading frames (ORF) and predicts protein coding sequences (PCS) on a sequence. The open reading frame finder in BIONUMERICS lists all ORFs and possible PCS regions for all six translation frames, for a given translation table and codon usage table. The user can specify an optional ORF and PCS length filter. ORFs and amino acid translations can be plotted on a graphical sequence display.

ORF prediction in the BIONUMERICS software

Primer design

The PCR primer design tool in the BIONUMERICS software searches for optimal primers or primer combinations for the most diverse experiment setups by taking into account a large number of experimental parameters. The user can specify various primer properties such as preferential length and melting temperature, %GC boundaries, maximal degeneracy, etc. Forward and reverse primers and PCR combinations can be sorted and selected according to different parameters (position, melting temperature, length, degeneracy, %GC...). Selected primer pairs can be stored in the oligonucleotide database.

Restriction enzyme analysis

In-silico multi-purpose analysis of restriction enzyme cleavage suitable for cloning experiments as well as for RFLP, PFGE and AFLP design. Thousands of restriction enzymes from ReBase can be downloaded and stored, and subsets of enzymes of particular interest can be created (e.g. 4-cutters, blunt-cutters, cheap enzymes, available enzymes...). Up to full chromosomes can be analyzed so that optimal enzymes can be selected for electrophoresis-based fragment typing techniques.