In-silico multi-purpose analysis of restriction enzyme cleavage suitable for cloning experiments as well as for RFLP, PFGE and AFLP design. Thousands of restriction enzymes from ReBase can be downloaded and stored, and subsets of enzymes of particular interest can be created (e.g. 4-cutters, blunt-cutters, cheap enzymes, available enzymes...). Up to full chromosomes can be analyzed so that optimal enzymes can be selected for electrophoresis-based fragment typing techniques. Individual enzyme cleavage fragments or combinations of different enzymes can be displayed on pseudo-gel images to predict the results of typing techniques or cloning experiments.