Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.

Delineating gel images

How important is the delineation of a gel by the green rectangle?

Compatible capillary sequencers

What automated sequencers is BioNumerics or GelCompar II compatible with?

Invalid TIFF file

When I open a TIFF file the program tells me "Invalid TIFF file". What is wrong?

Sample gel image file

Sample gel image of a set Restriction Fragment Length Polymorphism (RFLP) patterns, provided as a gray scale TIFF file.

AB sequencer trace files

Amplified Fragment Length Polymorphism (AFLP) profiles, ran on Applied Biosystems capillary electrophoresis equipment. The electropherograms are provided as .fsa files.

Quantification of bands

Is it possible to perform quantification studies in GelCompar II with the combination of the Basic Software and the Cluster Analysis module?

Data conversion old GelCompar

How do I convert my data from GelCompar 4.2 into BioNumerics or GelCompar II?


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