Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BIONUMERICS and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.
Fingerprints
Import and analyze your second gel
Edit fingerprint bands in the Comparison window
Create a tree from fingerprint data
Combined analysis of fingerprint data
Create and export a band matching
Import peak tables from automated sequencers
Import curve files from automated sequencers
Import and analyze your first gel
Combined analysis of fingerprint data
This tutorial illustrates how to create a dendrogram based on fingerprint data coming from different experiments, using a composite data set.