Band matching and polymorphism analysis

This tutorial illustrates how to create band matching tables. Band matching has a number of important applications such as screening for genetic markers (eg. using AFLP) in plant and animal breeding and microbial community analysis using PCR-DGGE, t-RFLP, ARISA or DHPLC.

Fingerprints

Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.

Download PDF file: 
Download demonstration database: 
DemoBase Connected

The DemoBase Connected demonstration database in BioNumerics version 7.6 format. This database is used in several tutorials and comes with the BioNumerics installation.

Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BioNumerics startup screen.

Share this: