Next-Generation Sequencing Workshop

NGS Workshop

After careful consideration of the continuing developments pertaining to the outbreak of the novel coronavirus (COVID-19), Applied Maths made the decision to postpone the Workshop set to take place April 22-24, 2020 in Ghent, Belgium. We share your disappointment in the cancellation of the event, but the safety, health and comfort of our attendees and staff is our highest priority.

Questions about the workshop can be send to We sincerely appreciate your continued support and look forward to seeing our workshop attendees at a future event.

During our two day Next-Generation Sequencing Workshop participants will get a good overview of all the possibilities of the BioNumerics software package for Next-Generation Sequencing (NGS) through numerous hands-on demos and exercises. By the end of the workshop you should have clear insights into the main BioNumerics NGS applications, including:
  • The import of NGS data as sequence read sets, including data validation and pre-processing,
  • The import of genomes and metadata from public repositories or other sources,
  • Mapping of reads against a reference genome,
  • De novo assembly,
  • Annotation of an assembled genome,
  • Whole genome MLST (wgMLST) analysis, i.e. gene-by-gene systems as an extension of traditional 7-gene MLST,
  • Whole genome SNP (wgSNP) analysis, i.e. the detection of SNPs on whole genomes sequences mapped against a reference sequence,
  • Phylogenetic analysis based on wgMLST and/or wgSNP, and
  • Genotyping tools that allow to screen the strain resistome and virulome, and in addition allow determination of e.g. pathotype (E. coli), serotype (E. coli), spa type (S. aureus)…

Detailed workshop agenda (PDF)

Analysis day

Optionally, you can register for an extra Analysis Day where you can bring your own data and work with our specialized staff on your individual analysis requirements.

Practical information

The Next-Generation Sequencing Workshop consists of two full days and will begin on Wednesday morning at 9am and runs until 5pm, with coffee breaks and a lunch break in between. A complimentary “networking” dinner is offered to all participants on Wednesday evening. On Thursday morning the sessions start again at 9am and end at 4pm. Workshop Notes are offered free of charge and include color-printed hand-outs of all the lectures and tutorials of the hands-on demonstrations and exercises. The Analysis Day starts at 9am until 1pm, with lunch also included.

A basic knowledge of the BioNumerics software or familiarity with the applications covered is presumed. For a more general introduction to the use of BioNumerics, we refer to our Basic Workshop.

The number of participants to the workshop is limited to 18. A maximum of 8 participants can be accommodated during the Analysis Day to ensure a high standard of quality and efficiency. In case the workshop is fully booked, the date of registration together with prepayment will be decisive. Applied Maths NV reserves the right to cancel this workshop up to one month before the scheduled date in case of insufficient registrations.

Registration fee

The registration for the general two-days Workshop, including 2 lunches and one evening dinner is 995 Euro (excl. VAT*). The cost for the Analysis Day on Friday is 545 Euro (excl. VAT*).


The Workshop and Analysis day will be held at the "Communication Loft" in Ghent (Sint-Denijslaan 485, Ghent). We will take you from the hotel to the workshop venue and back.


Rooms can be booked at the Best Western Hotel Chamade, at a special rate exclusively to workshop participants. The room rate is 103.50 Euro (incl. taxes) for a single room and 119 Euro (incl. taxes) for a double room, including breakfast and wireless internet in the room. In addition, a city tax of 3 Euro will be charged per person per night. The hotel is located at less than 10 minutes walking from the railway station. To make your reservation, please send your request to (subject:"block booking Applied Maths").

(*) For Belgian customers 21% VAT will be applied. For other customers within the EU please provide the VAT registration number of your organization to be exempted from paying the VAT. Federal and local taxes may apply for non-EU customers.

Date: Apr 22nd to 24th, 2020

Location: Ghent, BELGIUM

Please note that this workshop is canceled