MLST and wgMLST Workshop

This workshop is part of a series of thematic workshops on the use of BioNumerics for specific biological applications. The participants to the MLST and wgMLST Workshop get a good overview of all the possibilities of the BioNumerics software package for Multi-Locus Sequence Typing (MLST) and Whole Genome Multi-Locus Sequence Typing (wgMLST) through numerous hands-on demos and exercises. The main topics that will be covered in this training course include:

• Setup of the MLST online plugin with an interactive link to online repositories or for creating your own MLST schemes.
• Batch import and assembly of Sanger sequencer chromatograms into consensus sequences for the seven MLST loci.
• Automated assignment of alleles and profiles and quality check via the assembly.
• Setup of the wgMLST client plugin and use of the Calculation Engine for performing demanding wgMLST calculations in the cloud.
• Quality assessment of the wgMLST allele calls and comparison of the assembly-based and assembly-free algorithms.
• How to define and select different typing schemes in wgMLST, corresponding e.g. to core genome MLST (cMLST), ribosomal MLST (rMLST), “classical” MLST, etc.
• Clustering of MLST and wgMLST data using e.g. Minimum Spanning Trees.

The Workshop Notes include color-printed hand-outs of all the lectures and tutorials of the demonstrations and exercises.

Workshop hours are 9am to 5pm on Monday and 9am to 4pm on Tuesday. Lunch is included at 13:00h.

The workshop will be held in our training room in the Applied Maths facility in Sint-Martens-Latem, a quaint rural village at only 10 kilometers (6 miles) from the historical city center of Ghent. The registration cost per person for this thematic workshop, including 2 lunches and one evening dinner is 795 Euro (excl. VAT*).

Applied Maths can make hotel reservations (NH Hotel Gent Sint Pieters). The room rates are:

• 2015-05-10 - 2015-05-11: 99 Euro for a single room (supplement double room: 19.90 Euro)
• 2015-05-11 - 2015-05-13: 102 Euro for a single room (supplement double room: 19.90 Euro)

The room rate includes breakfast and wireless Internet. The city tax of 3.0 Euro per person is not included. The hotel is located at less than 10 minutes walking from the railway station. Bus transport from the hotel to the workshop facility and back is provided by Applied Maths.

Customers as well as non-customers who are interested in learning more about MLST and wgMLST are invited to attend this workshop. A basic knowledge of the BioNumerics software or familiarity with the applications covered is presumed. For a more general introduction to the use of BioNumerics, we refer to our Basic Workshops, which are organized several times per year.

To guarantee the quality and efficiency of the workshop, the maximum number of participants is limited to 24. In case the workshop is fully booked, the date of registration together with prepayment will be decisive. Applied Maths reserves the right to cancel this workshop up to one month before the scheduled date in case of insufficient response. Please do not hesitate to contact us if you would like to obtain more information.

(*) For Belgian customers 21% VAT will be applied. For other customers within the EU please provide the VAT registration number of your organization to be exempted from paying the VAT. Federal and local taxes may apply for non-EU customers.

Date: May 11^th to 12^th, 2015

Location: Sint-Martens-Latem, BELGIUM

Available places: 8