Quality assessment of next generation sequencing runs

This video shows how to assess the quality of imported next-generation sequencing runs in BioNumerics Seven. We look here at the number of GC bases, the average read quality distribution, the base distribution and the average quality distribution.

Sequence read sets

A sequence read set is designed to hold large sets of short reads generated by next generation sequencing (NGS). Base sequences and their associated quality scores are stored for single-end and paired-end reads, originating from various high-throughput pyrosequencing platforms such as Roche 454, Illumina Solexa, Ion Torrent, etc.

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