Whole genome SNP with mapping performed locally on your own computer

In this tutorial we will focus on the first two steps of the wgSNP workflow in BIONUMERICS: reference sequence selection and mapping of the reads against the reference sequence. The mapping of the reads against the reference sequence is done locally. 

wgSNP analysis

Whole-genome Single Nucleotide Polymorphism (SNP) analysis using BIONUMERICS. See the wgSNP application page for a complete overview.
Download PDF file: 
Download demonstration database: 
WGS_demo_database_for_Staphylococcus_aureus

Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.

Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.