Whole genome SNP with mapping performed locally on your own computer
In this tutorial we will focus on the first two steps of the wgSNP workflow in BIONUMERICS: reference sequence selection and mapping of the reads against the reference sequence. The mapping of the reads against the reference sequence is done locally.
wgSNP analysis
Whole-genome Single Nucleotide Polymorphism (SNP) analysis using BIONUMERICS. See the wgSNP application page for a complete overview.
Demonstration database containing data for a set of 97 Staphylococcus aureus isolates originating from three published studies. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.