Whole genome MLST typing in the Listeria monocytogenes demonstration database
A tutorial for working with the whole genome Multi Locus Sequence Typing (wgMLST) demonstration database of Listeria monocytogenes, which allows BioNumerics users to explore the possibilities of the wgMLST client plugin without the need for a wgMLST project or Calculation Engine credits.
wgMLST analysisWhole-genome Multi-Locus Sequence Typing (wgMLST) using BioNumerics. See the wgMLST application page for a complete overview.
Demonstration database containing data for a set of 51 Listeria monocytogenes isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BioNumerics startup screen.