Importing peak data from peak table files

This tutorial describes how to import band size and band position tables. During import fingerprints are reconstructed based upon the size and the amplitude (area or height) of the peaks.

Fingerprints

Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.

Download PDF file: 
Download sample data: 
VNTR sample peak table
Pooled multi-locus VNTR data, ran on a Beckman automated sequencer and subsequently converted into a peak table text file by the Beckman software.
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