Annotating sequences by Prokka

This tutorial demonstrates the annotation of genomes by Prokka[1] available on the Calculation Engine. 

[1] Torsten Seemann. Prokka: rapid prokaryotic genome annotation. Bioinformatics, 30(14):2068-2069, 2014.

Sequences

Nucleic acid or amino acid sequences, from import and assembly over multiple alignment to annotation, SNP analysis, primer design and comparison of complete chromosomes.

Download PDF file: 
Download demonstration database: 
WGS_demo_database_for_Salmonella_enterica

Demonstration database containing data for a set of 62 Salmonella enterica isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.

Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.