Delineating gel images

Question: 

How important is the delineation of a gel by the green rectangle?

Answer: 

It does not matter how you delineate the green rectangle while defining the gel contours. However, further normalization is easier when you try to define the green rectangle consistently: the corresponding bands will more precisely occur at the same position. A good trick is to look for a consistent band at the top of the lanes, and align the top of the rectangle to that band. The same for the bottom.

Package(s):

Applicable for: 
Version 1.0 - 7.6

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