A sequence read set is designed to hold large sets of short reads generated by next generation sequencing (NGS). Base sequences and their associated quality scores are stored for single-end and paired-end reads, originating from various high-throughput sequencing platforms such as Illumina, Ion Torrent, PacBio, Oxford Nanopore, etc.
This tutorial illustrates how to import FASTQ files into a BioNumerics database, how to obtain a quick overview of the basic statistics on read quality and read length, and finally how to perform a resequencing assembly against a reference sequence.
This tutorial illustrates how to import FASTQ files into a BioNumerics database, how to obtain a quick overview of the basic statistics on read quality and read length, and finally how to perform a de novo assembly locally so without using the external calculation engine.