Sequence read sets

A sequence read set is designed to hold large sets of short reads generated by next generation sequencing (NGS). Base sequences and their associated quality scores are stored for single-end and paired-end reads, originating from various high-throughput pyrosequencing platforms such as Roche 454, Illumina Solexa, Ion Torrent, etc.

Single sample diversity analysis on sequence read sets

This tutorial illustrates how to calculate diversity coefficients, starting from preprocessed sequence read sets.

Taxonomic identification on sequence read sets

This tutorial illustrates how to perform a taxonomic identification analysis, starting from preprocessed sequence read sets.

Sequence read sets: revealing the diversity of microbial populations

This tutorial illustrates the analysis of microbial population diversity, starting from the import of sequence read sets to the identification of operational taxonomic units and their abundances.

Importing FASTQ files and performing a de novo assembly

This tutorial illustrates how to import FASTQ files into a BioNumerics database, how to obtain a quick overview of the basic statistics on read quality and read length, and finally how to perform a de novo assembly.

Importing FASTQ files and linked database entry information (metagenomics)

This tutorial illustrates how to import multiplex identifier information from an Excel file, and how to import and link fastq files to these entries. This tutorial also illustrates how to obtain a quick overview of the basic statistics on read quality and read length, and how predefined charts are used to get a more indetailed view on these characteristics.

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