Fingerprints

Any type of data that can be translated into a densitometric curve is considered a fingerprint type in the BioNumerics and GelCompar II software. This includes commonly used genotyping methods employing agarose or polyacrylamide slab gel electrophoresis (PFGE, rep-PCR, RAPD, PCR-DGGE, etc.), in which case the data are usually imported as two-dimensional gel images (bitmaps). Another major group consists of capillary electrophoresis profiles such as AFLP, ARISA, T-RFLP, etc. Here, the raw electropherograms generated by an automated sequencer (genetic analyzer) or derived peak table text files can be imported. Finally, any other profile (generated e.g. by gas chromatography, HPLC or spectrophotometry) that can be seen as peaks or bands, can be analyzed as a fingerprint.

Bypass normalization

What does the option Bypass normalization in the fingerprint conversion settings imply?

Curve convertor maximum number of samples

Within the normalization step of the reference some of the samples were not normalized although the external references were assigned and the reference lanes were marked. Is there an upper limit of samples or are there other causes?

Curve convertor

Can you give me some information regarding the special functions within the CrvConv import program?

Maximum number of reference systems

What is the maximum number of reference systems allowed in one fingerprint type?

Unlinking a reference gel

In case of inline references, when you have a reference dye/gel linked to a data gel, can you unlink them once they are linked?

Internal reference positions

I am having trouble with the normalization of internal reference positions. After selecting Normalization > Update Normalization, the bands do not move and the arrows remain. Why do the internal reference positions not normalize?

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