BioNumerics is a very comprehensive software package, which has many applications in different research fields of the biological sciences. The listing below is by no means exhaustive, but gives an idea about what our software is capable of.
Multilocus sequence typing (MLST) is a technique whereby a number of housekeeping genes (loci) are sequenced, usually in part. In a typical MLST approach, recombination is expected to occur with a much higher frequency than point mutations. Therefore, one does not look at the total sequence similarity between strains. Instead, each sequence for a given locus is screened for identity with already known sequences for that locus. If the sequence is different, it is considered to be a new allele and is assigned a unique (arbitrary) allele number.
Inter-Simple Sequence Repeat Polymerase Chain Reaction (ISSR-PCR) is a reproducible and relatively inexpensive DNA fingerprinting method for animals, plants, fungi and bacteria. It is based on the amplification of genomic regions between microsatellite loci. Running this versatile typing method on an automated DNA sizing system such as the QIAxcel Advanced System reduces the number of manipulations required as well as the time to result. Raw data files from the QIAxcel Advanced System ScreenGel® software can be imported as densitometric curve files or peak table files in BioNumerics with the QIAxcel plugin.
BioNumerics software offers an integrated platform for the analysis of PFGE fingerprints. Store all your epidemiological info and gel images in one database. With powerful statistical tools, BioNumerics makes it easy to perform cluster analysis and calculate dendrograms.